<?xml version='1.0' encoding='UTF-8'?><?xml-stylesheet href="http://www.blogger.com/styles/atom.css" type="text/css"?><feed xmlns='http://www.w3.org/2005/Atom' xmlns:openSearch='http://a9.com/-/spec/opensearchrss/1.0/' xmlns:georss='http://www.georss.org/georss' xmlns:gd='http://schemas.google.com/g/2005' xmlns:thr='http://purl.org/syndication/thread/1.0'><id>tag:blogger.com,1999:blog-1544023933695049734</id><updated>2011-11-27T16:28:54.277-08:00</updated><category term='Protocol'/><category term='Biochemical Test'/><category term='Technique'/><title type='text'>Bio-technique</title><subtitle type='html'></subtitle><link rel='http://schemas.google.com/g/2005#feed' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/posts/default'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default?max-results=100'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/'/><link rel='hub' href='http://pubsubhubbub.appspot.com/'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><generator version='7.00' uri='http://www.blogger.com'>Blogger</generator><openSearch:totalResults>9</openSearch:totalResults><openSearch:startIndex>1</openSearch:startIndex><openSearch:itemsPerPage>100</openSearch:itemsPerPage><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-4257495849379328300</id><published>2010-12-30T00:45:00.000-08:00</published><updated>2010-12-30T00:45:30.705-08:00</updated><title type='text'>OXIDASE TEST</title><summary type='text'>DESCRIPTIONSome bacteria like Pseudomonas, Pasteurellaceae, Campylobacter, Neisseria, Moraxella, can produce cytochrome C oxidase located in their membrane which can catalyse  the transport of electrons from donor compounds to electron acceptors (oxygen). This respiratory system is present in aerobic bacteria which are capable to use oxygen as the final hydrogen receptor.


In the presence of </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/4257495849379328300/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/oxidase-test.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/4257495849379328300'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/4257495849379328300'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/oxidase-test.html' title='OXIDASE TEST'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-7241528497005358938</id><published>2010-12-26T23:26:00.001-08:00</published><updated>2010-12-26T23:26:45.142-08:00</updated><category scheme='http://www.blogger.com/atom/ns#' term='Technique'/><title type='text'>HLA Typing</title><summary type='text'>In 1975, Peter Doherty and Rolf Zinkernagel identified human leukocyte antigens (HLA). HLA are proteins found everywhere in the body except red blood cells. They are especially prevalent in white blood cells. Many types of HLA exist, often varying greatly from person to person. Doherty and Zinkernagel were awarded the 1996 Nobel Prize in Physiology or Medicine for this discovery.
Since many types</summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/7241528497005358938/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/hla-typing.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/7241528497005358938'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/7241528497005358938'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/hla-typing.html' title='HLA Typing'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-5355614109891369475</id><published>2010-12-26T23:25:00.000-08:00</published><updated>2010-12-26T23:25:12.507-08:00</updated><title type='text'>PCR Technique</title><summary type='text'>In 1983, Kary Mullis and members of the human genetics team at Cetus Corporation developed a genetic replication technique called polymerase chain reaction (PCR). After several years of fine-tuning the process, PCR became the most popular DNA replication technique by the 1990s. Kary Mullis was awarded the Nobel Prize in Chemistry for this work in 1993.
The popularity of PCR is based on both the </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/5355614109891369475/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/pcr-technique.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/5355614109891369475'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/5355614109891369475'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/pcr-technique.html' title='PCR Technique'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-2685915825784821340</id><published>2010-12-26T23:16:00.000-08:00</published><updated>2010-12-26T23:16:58.541-08:00</updated><category scheme='http://www.blogger.com/atom/ns#' term='Protocol'/><title type='text'>How to Use a Hemacytometer</title><summary type='text'>How to use a hemacytometer
by Heather Buschman
French physiologist Louis-Charles Malassez (1842-1909) studied a lot of things in his life. In dentistry, the residual cells of the epithelial root sheath in the periodontal ligament are now called the epithelial rests of Malassez. A genus of fungi is also named for him, which includes species that can cause dandruff and other skin infections.
</summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/2685915825784821340/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/how-to-use-hemacytometer.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/2685915825784821340'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/2685915825784821340'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/how-to-use-hemacytometer.html' title='How to Use a Hemacytometer'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><media:thumbnail xmlns:media='http://search.yahoo.com/mrss/' url='http://2.bp.blogspot.com/_lLkxRb0IDJ0/SjeSwCtoQkI/AAAAAAAAAE8/uA42hnfEuiQ/s72-c/equation.jpg' height='72' width='72'/><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-1585601801396390908</id><published>2010-12-26T23:13:00.000-08:00</published><updated>2010-12-26T23:13:43.145-08:00</updated><category scheme='http://www.blogger.com/atom/ns#' term='Technique'/><title type='text'>RFLP Technique</title><summary type='text'>

In 1985, Sir Alec Jeffreys developed restriction fragment length polymorphism (RFLP), which quickly became the standard technique for DNA testing throughout the 1980s. RFLP provided the world with the first form of genetic testing based on DNA, the body's genetic material.
Each person inherits a unique combination of DNA from both biological parents, and this DNA serves as the code for all of </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/1585601801396390908/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/rflp-technique.html#comment-form' title='1 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/1585601801396390908'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/1585601801396390908'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/rflp-technique.html' title='RFLP Technique'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>1</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-2363462355820118276</id><published>2010-12-26T06:12:00.000-08:00</published><updated>2010-12-26T06:12:09.579-08:00</updated><title type='text'>INDOLE PRODUCTION</title><summary type='text'>DESCRIPTION
Indole is an aromatic heterocyclic organic compound that can be produced by some bacteria as a degradation product of the amino acid tryptophan. Tryptophanase is the enzyme involved tryptophan degradation resulting indole, pyruvate, and ammonia. Indole test is most used for coliforms identification.
MATERIALS
Simplest medium for indole testing is Buffered peptone water (Proteose </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/2363462355820118276/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/indole-production.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/2363462355820118276'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/2363462355820118276'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/indole-production.html' title='INDOLE PRODUCTION'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-7667043581749700798</id><published>2010-12-26T04:37:00.000-08:00</published><updated>2010-12-26T04:37:42.525-08:00</updated><category scheme='http://www.blogger.com/atom/ns#' term='Biochemical Test'/><title type='text'>COAGULASE TEST</title><summary type='text'>DESCRIPTIONCoagulase is an enzyme produced by some bacteria that reacts with prothrombin in the blood, forming a complex named staphylothrombin, which causes blood to clot by converting fibrinogen to fibrin. This mechanism protect bacteria from phagocytosis. Frequently coagulase test is used to distinguish between different types of Staphylococcus isolates.

PROCEDUREHarvest blood from  a rabbit </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/7667043581749700798/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/coagulase-test.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/7667043581749700798'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/7667043581749700798'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/coagulase-test.html' title='COAGULASE TEST'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-3830969824514746093</id><published>2010-12-26T04:31:00.000-08:00</published><updated>2010-12-26T04:31:28.961-08:00</updated><title type='text'>Types of microscopes</title><summary type='text'>There are several types of microscopes available on the market, selection of the proper type is not a simple assignmen as you would need to determine what exactly it would be used for. Below you can see all the types of modern microscopes for any scientific and hobby task.


A compound microscope is an optical device made for magnifying objects, consists of a number of lenses forming the image by</summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/3830969824514746093/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/types-of-microscopes.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/3830969824514746093'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/3830969824514746093'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/types-of-microscopes.html' title='Types of microscopes'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry><entry><id>tag:blogger.com,1999:blog-1544023933695049734.post-6597379566495278178</id><published>2010-12-26T04:02:00.000-08:00</published><updated>2010-12-26T04:02:53.401-08:00</updated><title type='text'>Bacteria - Identifying And Classifying Bacteria</title><summary type='text'>The most fundamental technique for classifying bacteria is the gram stain, developed in 1884 by Danish scientist Christian Gram. It is called a differential stain because it differentiates among bacteria and can be used to distinguish among them, based on differences in their cell wall.
In this procedure, bacteria are first stained with crystal violet, then treated with a mordant—a solution that </summary><link rel='replies' type='application/atom+xml' href='http://biotechnique.blogspot.com/feeds/6597379566495278178/comments/default' title='Post Comments'/><link rel='replies' type='text/html' href='http://biotechnique.blogspot.com/2010/12/bacteria-identifying-and-classifying.html#comment-form' title='0 Comments'/><link rel='edit' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/6597379566495278178'/><link rel='self' type='application/atom+xml' href='http://www.blogger.com/feeds/1544023933695049734/posts/default/6597379566495278178'/><link rel='alternate' type='text/html' href='http://biotechnique.blogspot.com/2010/12/bacteria-identifying-and-classifying.html' title='Bacteria - Identifying And Classifying Bacteria'/><author><name>biotechnology</name><uri>http://www.blogger.com/profile/12789946794166667023</uri><email>noreply@blogger.com</email><gd:image rel='http://schemas.google.com/g/2005#thumbnail' width='16' height='16' src='http://img2.blogblog.com/img/b16-rounded.gif'/></author><thr:total>0</thr:total></entry></feed>
